Safety measures in microbiological laboratory

Work in microbiological laboratory demands strict observance of special rules that is defined by two substantive provisions.

The first - in microbiological practice is used, mainly, pure cultures of microorganisms, i.e. populations of microorganisms of one kind which is often being posterity of one cell.

As in air, on a surface of subjects surrounding us, on clothes, hands, hair always there is a considerable quantity of various microflora for maintenance of sterility of researches and avoidance of pollution of cultures work should be spent with observance of aceptic rules.

The second - at researches with not identified microorganisms, at their allocation from objects of environment and technogenic streams, can be allocated pathogenic and conditionally pathogenic microorganisms.

Besides, cells of saprophytes and pathogenic microorganisms can be allergens for certain individuals. Thus, for reception of authentic results of researches, for maintenance of a personal security necessary and safety of associates observance of certain rules.

Preparation of a premise for carrying out of microbiological works includes wet cleaning and careful ventilation with the subsequent irradiation ultra-violet beams of bactericidal lamps. A table surface where work with cultures of microorganisms is directly spent, it is necessary to disinfect by grinding of 3 % a solution of chloramin or by 70 % solution of isopropil or ethyl spirits.

Preparation to laboratory works is spent by the laboratorian; students, carrying out of the task, should observe following rules:

1. Each student in microbiological laboratory works on a constant place, carrying out of the task individually.

2. On a workplace there should not be extraneous subjects (including portfolios and bags).

3. The student should work only in pure dressing gowns, hair should be picked up, not fall on shoulders.

4. At work with cultures of microorganisms it is necessary to observe all rules of microbiological technics. On test tubes, flasks, cups of Petri, mattresses should be made the inscription containing patrimonial and specific names of culture, dates of planting, a surname of the student and group number.

5. All subjects used at work with live cultures, should be disinfected by burning in a torch flame (loops, needles), or are shipped in a disinfectant solution (subject and integumentary glasses, pipettes).

6. All sowed test tubes, cups will go mad in the thermostat or surrender to the laboratorian. The fulfilled material (test tubes, Petri dishes) also is located in certain capacities under instructions of the laboratorian for their further disinfecting.

7. In laboratory smoking, food intake, superfluous circulation on laboratory are forbidden.

8. In the end of employment the student should put in order a workplace, wash up hands. It is necessary to have an individual towel, napkins for a wipe of hands. Each student conducts the magazine of laboratory works which is the document, allowing to supervise correctness of the received data. Records are spent in certain sequence and should contain the following:

1. Work number. Its name. Date of statement and the experience termination.

2. Object of research.

3. Conditions of carrying out of experience, including methods of analyses.

4. The received results and conclusions from them.

Laboratory work №1